BSN-200

  • 全流程深低温保护
  • 菜单操作页面 简易友好
  • 单支存取 独立单元存储
  • 机械装置常温工作 运行精准可靠
索取资料

BSN-200全自动深低温生物样本存储系统,是一款专为保藏活体生物样本而设计的智能化、自动化、蜂巢式的深冷存储设备可提供-196℃气相液氮存储环境,存储量约18800支(2ml)。

出色的自动化机械装置和智能化管理系统,避免了人工操作的失误,确保样本存取安全、高效、稳定、可追溯,全方位保障样本安全,有效规避样本资源的重大损失。


机械装置处于常温状态,运行精准可靠

样本存取区域精准降温,机械装置处于常温状态,运行精度可靠,设备使用寿命延长。


全流程深低温保护,防止反复冻融

样本的提取、转移、扫描、寻位、置入等各环节实现玻璃化温度以下的闭环深低温保护,防止反复冻融,保障样本活性。


独立单元存储,避免交叉干扰

蜂巢式结构设计,可实现独立单元存储,可分区域管理,避免交叉干扰,空间利用率高,使存储区域的深低温环境更加均衡稳定。


高效除湿净化,防霜冻

独特的双门密封传递窗和干燥净化系统设计,有效避免湿气和杂质进入设备内仓,防止设备内部工作及存储区域结霜结冻。


智能化数据管理 信息可追溯

BSN-Cyber操作管理系统。可自动分解和下达各项需求指令,实时监控设备运行环境及安全参数,存储空间碎片化整理,对样本高效检索,可预约操作。还能全线追溯样本信息,内置自动多级报警装置,确保样本存储和操作过程安全可靠。




外形尺寸

设备尺寸

1500(W)×2280(D)×2300(H)mm

设备重量(空重)

2100kg (4630 lbs)

存储能力

存储容量

48700 支(2ml

存储结构

蜂巢式合金管

样本保存方式

冻存管保存温度

-150

冻存管保存环境

气相液氮环境

操作方式

触摸屏/PC

15in(触摸屏尺寸)

液氮消耗

静态液氮消耗量

15-20kg/24h

功率

电压/频率/电流

220V / 50HZ / 16A

待机功率

150W

最大功率

750W


Temperature fluctuations during deep temperature cryopreservation reduce PBMC recovery, viability and T-cell function

AnjaGermanna,Young-JooOha,TommSchmidta,UweSchöna,HeikoZimmermanna,b,Hagenvon Briesenaa

aFraunhofer Institute for Biomedical Engineering, Ensheimerstr. 48, 66386 St. Ingbert, Germany
bLehrstuhl für Molekulare und Zelluläre Biotechnologie/Nanotechnologie, Universität des Saarlandes, 66123 Saarbrücken, Germany

Abstract

The ability to analyze cryopreserved peripheral blood mononuclear cell (PBMC) from biobanks for antigen-specific immunity is necessary to evaluate response to immune-based therapies. To ensure comparable assay results, collaborative research in multicenter trials needs reliable and reproducible cryopreservation that maintains cell viability and functionality. A standardized cryopreservation procedure is comprised of not only sample collection, preparation and freezing but also low temperature storage in liquid nitrogen without any temperature fluctuations, to avoid cell damage. Therefore, we have developed a storage approach to minimize suboptimal storage conditions in order to maximize cell viability, recovery and T-cell functionality.

We compared the influence of repeated temperature fluctuations on cell health from sample storage, sample sorting and removal in comparison to sample storage without temperature rises. We found that cyclical temperature shifts during low temperature storage reduce cell viability, recovery and immune response against specific-antigens. We showed that samples handled under a protective hood system, to avoid or minimize such repeated temperature rises, have comparable cell viability and cell recovery rates to samples stored without any temperature fluctuations. Also T-cell functionality could be considerably increased with the use of the protective hood system compared to sample handling without such a protection system.

This data suggests that the impact of temperature fluctuation on cell integrity should be carefully considered in future clinical vaccine trials and consideration should be given to optimal sample storage conditions.