BSN 500

  • 使用液氮来进行深低温保存
  • 生物样本的活性可以保持非常长的时间
  • 提供完善的自动化解决方案
  • 避免手动进行样本存储和管理的低效和易于出错
索取资料

超大容量

本型号采用四罐独立存储的模块化结构形式,利于样本分组独立存储管理,并提升样本的安全可靠性;

高效地工作

采用五轴自动化结构的全自动运行机构设计,运行可靠高效;存储罐盖自动开盖设计,运行更快;

真空负压冻存管取放机构设计,运行更加可靠,维护更加便捷;

高干燥度的除湿系统,防止设备内部工作区域及存储区域结霜结冻;

样本的安全

冻存管提取装置深低温全冷链保护,确保样本安全;

高效的二维码扫描系统,可快速实现样本信息的核对确认;

全自动样本传递窗设计,自动进出,自动除湿,并可方便实现与AGV系统的全方位对接;

配备UPS电源,更加确保样本的安全可靠;采用服务器信息存储,数据信息保存更加可靠安全;

简单易用

采用独特的BSN-Cyber操作管理系统,无论是单台、多台均可轻松操作和管理;

嵌入式触摸屏倾角显示,方便人机对话和操作;

全面的系统信息反馈和异常信息报警系统设计,确保各种异常情况不会影响到样本安全;

可实现紧急情况的存储模块的快速转运或转移。

BSN500全自动深低温生物样本存储设备参数表


设备尺寸
1.5(W)×2.28(D)×2.3(H)m
设备重量
2500kg (5,500 lbs)
适用样本容积
2mL
液氮罐保温时间(低液位报警)
72小时
冻存管保存温度
-160℃以下
存储容量
50,000
触摸屏尺寸
12英寸
中转罐尺寸
φ256×230
静态液氮消耗量
23kg/
液氮连接方式
3/4-16UNF
电压/频率/电流
220V 50/50HZ 16A
待机功率
210W,最大功率600W




Temperature fluctuations during deep temperature cryopreservation reduce PBMC recovery, viability and T-cell function

AnjaGermanna,Young-JooOha,TommSchmidta,UweSchöna,HeikoZimmermanna,b,Hagenvon Briesenaa

aFraunhofer Institute for Biomedical Engineering, Ensheimerstr. 48, 66386 St. Ingbert, Germany
bLehrstuhl für Molekulare und Zelluläre Biotechnologie/Nanotechnologie, Universität des Saarlandes, 66123 Saarbrücken, Germany

Abstract

The ability to analyze cryopreserved peripheral blood mononuclear cell (PBMC) from biobanks for antigen-specific immunity is necessary to evaluate response to immune-based therapies. To ensure comparable assay results, collaborative research in multicenter trials needs reliable and reproducible cryopreservation that maintains cell viability and functionality. A standardized cryopreservation procedure is comprised of not only sample collection, preparation and freezing but also low temperature storage in liquid nitrogen without any temperature fluctuations, to avoid cell damage. Therefore, we have developed a storage approach to minimize suboptimal storage conditions in order to maximize cell viability, recovery and T-cell functionality.

We compared the influence of repeated temperature fluctuations on cell health from sample storage, sample sorting and removal in comparison to sample storage without temperature rises. We found that cyclical temperature shifts during low temperature storage reduce cell viability, recovery and immune response against specific-antigens. We showed that samples handled under a protective hood system, to avoid or minimize such repeated temperature rises, have comparable cell viability and cell recovery rates to samples stored without any temperature fluctuations. Also T-cell functionality could be considerably increased with the use of the protective hood system compared to sample handling without such a protection system.

This data suggests that the impact of temperature fluctuation on cell integrity should be carefully considered in future clinical vaccine trials and consideration should be given to optimal sample storage conditions.