BSN 500

Automated Extra-Large-Capacity Biobanking

  • Use liquid nitrogen for deep and low temperature preservation.
  • The activity of biological samples can be maintained for a very long time.
  • Provide complete automation solutions.
  • Avoid the inefficiencies and pitfalls of manually conducting sample storage and management.
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The model is engineered by following innovative designs:

using four independent freezers structure can make better sample grouping storage management and can enhance the safety and reliability of the sample;

using full automatic operation mechanism design with a five-shaft automatic structure, can ensure a higher efficiency and more reliable operation.;

advanced automatic lid opening design can lead to faster operation;

vacuum and negative pressure sample pick-up mechanism design can secure more reliable operation and more convenient maintenance;

high dryness dehumidification system can prevent frost in working area and frozen inside the storage area;

special cryogenic protection pick-up device can ensure the little exposure of frozen sample;

integrated two-dimensional code scanning system can realize high efficient sample information verification;

comprehensive automatic transfer window design with the functions of automatic dehumidifying and automatic import and export can easily equip with advanced AGV system;

installed with UPS can ensure uninterrupted operation;

introducing server to store the information can ensure more reliable and safety data storage;

the intelligent BSN-Cyber operation system can be easy to use on both single and multiple modular;

embedded touch screen with obliquity display can have more convenient conversational interface;

comprehensive system design for information feedback and abnormal alarm ensure all kinds of abnormal situation will not affect the safety of the sample; easy removable module design can realize emergency rapid transfer of samples.

Item Specification
Model BSN-500
Storage Capacity 50000(2.0ml Vials)
Dimension (mm) 1700×2280×2420(Width×Depth×Height)
Weight (empty) 2500KG
Weight (full capacity) 2800KG
Power Standby:<500w,Max operating power:<2500w
Voltage 220V,50Hz
Hose Interface (mm) Ø15mm
Touch Screen 12 Inch
LN2 Evaporation Rate (L/day) 20(Static)
Freezer hold time(≤130℃) ≥48h
Storage Temperature ≤-160℃
Freezer Dimension (mm) * φ600*1470mmx4
*BSN-500 has a special four freezers input design

Temperature fluctuations during deep temperature cryopreservation reduce PBMC recovery, viability and T-cell function

AnjaGermanna,Young-JooOha,TommSchmidta,UweSchöna,HeikoZimmermanna,b,Hagenvon Briesenaa

aFraunhofer Institute for Biomedical Engineering, Ensheimerstr. 48, 66386 St. Ingbert, Germany
bLehrstuhl für Molekulare und Zelluläre Biotechnologie/Nanotechnologie, Universität des Saarlandes, 66123 Saarbrücken, Germany

Abstract

The ability to analyze cryopreserved peripheral blood mononuclear cell (PBMC) from biobanks for antigen-specific immunity is necessary to evaluate response to immune-based therapies. To ensure comparable assay results, collaborative research in multicenter trials needs reliable and reproducible cryopreservation that maintains cell viability and functionality. A standardized cryopreservation procedure is comprised of not only sample collection, preparation and freezing but also low temperature storage in liquid nitrogen without any temperature fluctuations, to avoid cell damage. Therefore, we have developed a storage approach to minimize suboptimal storage conditions in order to maximize cell viability, recovery and T-cell functionality.

We compared the influence of repeated temperature fluctuations on cell health from sample storage, sample sorting and removal in comparison to sample storage without temperature rises. We found that cyclical temperature shifts during low temperature storage reduce cell viability, recovery and immune response against specific-antigens. We showed that samples handled under a protective hood system, to avoid or minimize such repeated temperature rises, have comparable cell viability and cell recovery rates to samples stored without any temperature fluctuations. Also T-cell functionality could be considerably increased with the use of the protective hood system compared to sample handling without such a protection system.

This data suggests that the impact of temperature fluctuation on cell integrity should be carefully considered in future clinical vaccine trials and consideration should be given to optimal sample storage conditions.